HPLC & GC Columns

1. Choose columns based on functions:

Normal phase: solvent soluble solutions
Reverse phase: hydrocarbon soluble solutions
Ion-exchange: separate monosaccharides
Size-exclusion: separate proteins and carbohydrates


2. Diameter x Length vs Pressure:

Shorter and wider columns cut analysis time and require less pressure to move the mobile phase.
Longer and narrower columns provide higher resolution and more sensitive results. They require less samples for analysis but a higher pressure.

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3. Column Material:

Stainless steel is the material of choice for packed columns in high pressure (HPLC) analysis
Capillary columns are much thinner because the stationary phase is chemically bonded to their inner wall coating.
We recommend wall-coated open tubular (WCOT) capillary columns with fused silica lining tubes for gas chromatography (CG). Because of their superior strength and flexibility.

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